Proteinase K (recombinat PCR grade) (Roche Diagnostics, catalog number: 03 115 887 001 ) GlycoBlue TM Coprecipitant (15 mg/ml) (Life Technologies, catalog number: AM9515 ) Prestained Protein Ladder (broad range) (New England Biolabs, catalog number: P7710S )
T4 Polynucleotide Kinase (10 U/µl) (Thermo Fisher Scientific, catalog number: EK0031 )Ĥx NuPAGE ® LDS Sample Buffer (Life Technologies, catalog number: NP0007 )Ģ0x NuPAGE ® MOPS SDS Running Buffer (Life Technologies, catalog number: NP0001 ) Γ-P32-ATP (10 ♜i/µl) (HARTMANN ANALYTIC GmbH, catalog number: SCP-401 ) Note: The RNA is delivered as 5 nmol aliquots, stored at -80 ☌ and dissolved in 25 µl MQ-water before usage. RNasin (Dundee Cell Products, catalog number: RS3100 )ģ’ RNA linker (5'-P-UGAGAUCGGAAGAGCGGUUCAG-Puromycin-3') (Microsynth AG ) T4 RNA Ligase (10 U/µl) (Thermo Fisher Scientific, catalog number: EL0021 ) Goat anti-RENT1 antibody (Bethyl Laboratories, catalog number: A300-038A ) (UPF1 is also named RENT1.)ĭynabeads ® Protein G (Life Technologies, catalog number: 10004D )Īlkaline Phosphatase Calf Intestinal (CIP) (New England Biolabs, catalog number: M0290S ) Turbo DNase (Life Technologies, Ambion ®, catalog number: AM2238 ) RNase I (cloned) (100 U/µl) (Life Technologies, Ambion ®, catalog number: AM2294 ) Trypsin-EDTA (T/E) (BioConcept, Amimed, catalog number: 5-5iF00-H )ĭEPC BioChemica (AppliChem GmbH, catalog number: A0881, 0250 )ġ00x Halt TM Protease Inhibitor Cocktail (Thermo Fisher Scientific, catalog number: 1861279 ) Penicillin-Streptomycin solution (P/S) (1 unit/ml) (BioConcept, Amimed, catalog number: 4-01F00-H ) DMEM (powder, high glucose) (Life Technologies, catalog number: 52100-039 )įetal Calf Serum (FCS) (BioConcept, Amimed, catalog number: 2-01F30-I )
0 kommentar(er)
